Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

SRP3128

Sigma-Aldrich

NT-3 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Sinonimo/i:

HGNF, NGF-2, Nerve growth factor-2, Neurotrophic factor, Neurotrophin-3

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in E. coli

Saggio

≥98% (HPLC)
≥98% (SDS-PAGE)

Forma fisica

lyophilized

Potenza

≤20 ng/mL ED50/EC50

PM

13.6 kDa

Confezionamento

pkg of 10 μg

tecniche

cell culture | mammalian: suitable

Impurezze

<0.1 EU/μg endotoxin, tested

Colore

white to off-white

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... NTF3(4908)

Descrizione generale

NT-3 (neurotrophin 3) is a neurotrophic factor structurally related to β-NGF (nerve growth factor), BDNF (brain-derived neurotrophic factor), and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues). The amino acid sequences of human, murine and rat NT-3 are identical.

Azioni biochim/fisiol

NT-3 (neurotrophin 3) promotes the growth and survival of nerve and glial cells. It is associated with neuroprotection and polymorphisms in the gene are associated with risk to Alzheimer′s disease. It is also linked with attention-deficit/hyperactivity disorder (ADHD) and mood disorders. NT-3 enhances the metastatic proliferation of breast cancer cells in the brain.

Sequenza

MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT

Stato fisico

Lyophilized with no additives.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Neurotrophin 3 genotype and emotional adverse effects of osmotic-release oral system methylphenidate (OROS-MPH) in children with attention-deficit/hyperactivity disorder.
Park S, et al.
Journal of Psychopharmacology, 28, 220-226 (2014)
Polymorphisms of the neurotrophic factor-3 (NTF-3) in Alzheimer's disease: rs6332 associated with onset time and rs6489630 T allele exhibited a protective role.
Liu M, et al.
Journal of Neurogenetics, 29, 183-187 (2015)
S O Meakin et al.
Trends in neurosciences, 15(9), 323-331 (1992-09-01)
The neurotrophins, of which nerve growth factor (NGF) is the best known example, support the survival and differentiation of chick embryo sensory neurons at extremely low concentrations, 10(-12) M or less. These same neurons display two different classes of neurotrophin
Neurotrophin-3 modulates breast cancer cells and the microenvironment to promote the growth of breast cancer brain metastasis.
Louie E, et al.
Oncogene, 32, 4064-4077 (2013)
The neurotrophin family of neurotrophic factors: an overview.
Skaper SD
Methods in Molecular Biology, 846, 1-12 (2012)

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.