SAE0202
Mucinase StcE
mass spec suitable, from EHEC, recombinant, powder
Sinonimo/i:
Mucin-specific protease StcE, secreted protease of C1 esterase inhibitor
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Descrizione generale
Mucinase StcE is a metalloprotease that can be used to digest densely O-glycosylated mucins. The consensus sequence for cleavage is S/T*-X-S/T, where the cleavage happens on the amino side of the 2nd Serine/Threonine, and X can be any amino acid, or none though with lower efficiency. The P2 position (*) is found to be always occupied in cleavage products, and occupancy of both P1 and P1’ positions do not interfere with cleavage. While Mucinase StcE is active against Mucins and other mucin-domain containing glycoproteins such as CD43 or PSGL-1, it is not active against O-glycoproteins outside of the Mucin family, such as fetuin or GspB736flag1.
Applicazioni
For use as a tool in biochemical research and biomarker discovery, particularly focused on glycobiology, glycoproteomics, glyco-oncology. Shown to be useful in LC-MS/MS workflows
Azioni biochim/fisiol
Mucins are heavily O-glycosylated proteins found in many tissues such as mucosal membranes and intestinal linings. One of its functions is as a protective layer for the epithelial tissue. The pathogenic bacteria enterohemorrhagic Escherichia coli (EHEC) is known to attack these tissues by enzymatic means. One of the enzymes found to be involved is a protease called secreted protease of C1 esterase inhibitor (StcE) (a.k.a. Mucinase StcE). This enzyme specifically recognizes densely glycosylated mucins and mucin-domains, and proteolytically cleaves the peptide backbone, presumably enhancing the virulence of the pathogenic microbe.
Caratteristiche e vantaggi
- Protease highly selective to mucins, but promiscuous within mucin family.
- Tool for O-linked glycopeptide release
- Configured in packages useful for approximately 40 samples
- His-Tag fusion for easy purification and isolation
Stoccaggio e stabilità
The product retains activity for at least 2 years when stored lyophilized at -20°C
Altre note
Solution of StcE can be prepared by reconstitution of the lyophilized 20 µg vial in 100 µL of water or PBS. The resuspended protein solution is stable for 2 weeks at 2-8 °C. For longer storage, aliquot the protein solution and store at -20 °C. The enzyme solution should be protected from direct light.
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.