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Key Documents

S6197

Sigma-Aldrich

AntiSTIM1 (C-terminal)

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

AntiGOK, AntiStromal interaction molecule 1

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen 90 kDa

Reattività contro le specie

human, rat, mouse

Concentrazione

~1 mg/mL

tecniche

immunoprecipitation (IP): 2.5-5 μg using extracts of rat PC12 cells.
indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells.
western blot: 2-4 μg/mL using whole extract of mouse 3T3 cells.

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... STIM1(6786)
mouse ... Stim1(20866)
rat ... Stim1(117086)

Descrizione generale

Stromal interaction molecule 1 (STIM1) is a conserved single-pass transmembrane protein. STIM1 is expressed ubiquitously in a wide variety of human primary and transformed cell types. STIM1 localizes predominantly in the membrane of the endoplasmic reticulum (ER). It contains an N-terminal EF hand motif located in the ER lumen and appears to function as a sensor of ER Ca2+ levels.

Immunogeno

synthetic peptide corresponding to amino acids 657-683 of human STIM1, conjugated to KLH via an N-terminal residue. The corresponding sequence is identical in rat, mouse, bovine, monkey, and dog.

Applicazioni

Anti-STIM1 (C-terminal) antibody produced in rabbit has been used in:
  • western blot
  • immunoprecipitation
  • immunofluorescence
  • immunohistochemistry

Azioni biochim/fisiol

Stromal interaction molecule 1 (STIM1) is required for the activation of store-operated Ca2+ influx. STIM1 undergoes rapid oligomerization and redistributes into discrete spots (punctae) that move towards and accumulate in the cell periphery, possibly to activate Orai1 that is located in the plasma membrane. Overexpression of STIM1 and Orai1 together markedly increases the calcium release-activated channels (CRAC) current (I-CRAC). Dynamic assembly of TRPC1-STIM1-Orai1 ternary complex plays a major role in the activation of store-operated calcium (SOC) channel in response to internal Ca2+ store depletion.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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miR-424/322 regulates vascular smooth muscle cell phenotype and neointimal formation in the rat
Merlet E, et al.
Cardiovascular Research, 98(3), 458-468 (2013)
Elise Merlet et al.
Cardiovascular research, 98(3), 458-468 (2013-03-01)
Our aim was to identify new microRNAs (miRNAs) implicated in pathological vascular smooth muscle cells (VSMCs) proliferation and characterize their mechanism of action. MicroRNAs microarray and qRT-PCR results lead us to focus on miR-424 or its rat ortholog miR-322 (miR-424/322).
Zhen-Dong Zhu et al.
Cell death & disease, 9(2), 50-50 (2018-01-21)
Acute pancreatitis (AP) is an acute inflammatory process of the pancreas that is characterized by inflammation, edema, vacuolization and necrosis, which has significant morbidity and lethality. The pathogenesis of AP has not been established completely. An early and critical feature
STIM1 is a microtubule plus end tracking protein involved in remodeling of the endoplasmic reticulum
Grigoriev I, et al.
Current Biology, 18(3), 177-177 (2008)
Cooperative Binding of Stromal Interaction Molecule 1 (STIM1) to the N and C Termini of Calcium Release-activated Calcium Modulator 1 (Orai1)
Palty R, et al.
The Journal of Biological Chemistry, 291(1), 334-341 (2016)

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