P6278
Peroxidase Inactivated
≤0.02 units/mg protein (biuret)
Sinonimo/i:
Peroxidase, inactivated from horseradish peroxidase
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About This Item
Prodotti consigliati
Stato
powder
Livello qualitativo
200
300
Attività specifica
≤0.02 units/mg protein (biuret)
Impurezze
salt, free
Solubilità
0.85% sodium chloride: soluble 15 mg/mL, clear to slightly hazy, colorless to light yellow
Temperatura di conservazione
2-8°C
Categorie correlate
Applicazioni
Inactivated peroxidase is used to study regeneration of inactivated peroxidase enzymes. It has been used to study the mechanism of peroxidase solubilization and it is used to study antitumor activity of immobilized peroxidase systems. It may be used for developing differential staining techniques in nerve tissue preparations.
Azioni biochim/fisiol
HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods that include the use of glutaraldehyde, periodate oxidation, disulfide bonds, and also via amino- and thiol-directed cross-linkers. It is smaller and more stable than the enzyme labels, β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and ions such as Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ are found to inhibit the enzyme activity.
Inactivated peroxidase reacts with goat and rabbit anti-peroxidase. It may be reactivated various methods such as heating.
Definizione di unità
One unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20°C.
Nota sulla preparazione
Prepared from Type VI horseradish peroxidase by a modification of the method of Teale.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Cleavage of the haem-protein link by acid methylethylketone.
F W TEALE
Biochimica et biophysica acta, 35, 543-543 (1959-10-01)
REGENERATION OF HEAT-INACTIVATED PEROXIDASE
Sigmund Schwimmer
The Journal of Biological Chemistry, 154, 487-495 (1944)
Antitumor Activity of an Immobilized Peroxidase System Against Murine Ehrlich Ascites Is Mediated by the Immune System
Steven H. Combs, Clinton D. Morgan, et al.
Exp. Biol. Med, 204, 306-311 (1993)
C J Forehand et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 2(5), 654-652 (1982-05-01)
This study of spinal cord injury in bullfrog (Rana catesbeiana) tadpoles using the neuroanatomical tracer horseradish peroxidase (HRP) was undertaken to determine (1) whether the same anatomical regions that normally give rise to ascending or descending spinal tracts do so
Dual projections of single neurons are visualized simultaneously: use of enzymatically inactive [3H]HRP.
N L Hayes et al.
Brain research, 165(2), 321-326 (1979-04-13)
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