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P1944

Sigma-Aldrich

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

Sinonimo/i:

phenol chloroform RNA extraction

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About This Item

Codice UNSPSC:
12190000
NACRES:
NA.52

Grado

for molecular biology

Livello qualitativo

200

Saggio

≥99.0%

Forma fisica

liquid

pH

4.3-4.7

applicazioni

life science and biopharma

Temperatura di conservazione

2-8°C

Descrizione generale

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

Applicazioni

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
  • for the extraction and purification of RNA.
  • to isolate RNA from Arabidopsis siliques.
  • to extract proteins and DNA from polysomal and non-polysomal fractions.
  • in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Organi bersaglio

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

175.0 °F - closed cup

Punto d’infiammabilità (°C)

79.44 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Phenol:Chloroform:Isoamyl Alcohol OmniPur® Grade, 25:24:1 ratio, phenol: >=99.0%, Suitable for DNA applications

Millipore

6805-OP

Phenol:Chloroform:Isoamyl Alcohol

Fenolo BioUltra, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

77608

Fenolo

Chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Sigma-Aldrich

25668

Chloroform – isoamyl alcohol mixture

Chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 24:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Supelco

25666

Chloroform – isoamyl alcohol mixture

Cloroformio contains 100-200 ppm amylenes as stabilizer, ≥99.5%

Sigma-Aldrich

C2432

Cloroformio

Cloroformio biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Sigma-Aldrich

496189

Cloroformio

Fenolo BioUltra, for molecular biology, TE-saturated, ~73% (T)

Sigma-Aldrich

77607

Fenolo

Small untranslated RNA antitoxin in Bacillus subtilis.
Silvaggi JM
Journal of Bacteriology, 187(19), 6641-6650 (2005)
An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
C Puissant et al.
BioTechniques, 8(2), 148-149 (1990-02-01)
C Pistore et al.
Oncogene, 36(40), 5551-5566 (2017-06-06)
Widespread genome hypo-methylation and promoter hyper-methylation of epithelium-specific genes are hallmarks of stable epithelial-to-mesenchymal transition (EMT), which in prostate cancer (PCa) correlates with castration resistance, cancer stem cells generation, chemoresistance and worst prognosis. Exploiting our consolidated 'ex-vivo' system, we show
Defining transcribed regions using RNA-seq.
Wilhelm BT
Nature Protocols, 5(2), 255-266 (2010)
PCR inhibitor levels in concentrates of biosolid samples predicted by a new method based on excitation-emission matrix spectroscopy.
Rock C
Applied and Environmental Microbiology, 76(24), 8102-8109 (2010)
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