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Key Documents

P1484

Sigma-Aldrich

Anti-p21WAF1/Cip1antibody, Mouse monoclonal

clone CP74, purified from hybridoma cell culture

Sinonimo/i:

Anti-CDKN1A, Anti-Cip1, Anti-WAF1, Anti-p21

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

CP74, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen 21 kDa

Reattività contro le specie

mouse, human

Concentrazione

~1 mg/mL

tecniche

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using LNCap cells (human prostate adenocarcinoma) treated with MG132 proteosome inhibition.

Isotipo

IgG2b

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CDKN1A(1026)
mouse ... Cdkn1a(12575)

Descrizione generale

Monoclonal Anti-p21WAF1/Cip1 (mouse IgG2b isotype) is derived from the CP74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant human p21WAF1/Cip1. p21WAF1/Cip1 is 21 kDa and designated as Cip1, WAF1, Sdi1, Pic1, CAP20.
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody reacts specifically with p21WAF1/Cip1. Two families of CKIs have been identified. The p21WAF1/Cip1 family contains p21WAF1/Cip1, p27Kip1 and p57Kip2.

Immunogeno

recombinant human p21WAF1/Cip1.

Applicazioni

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody may be used for ELISA, immunoblotting (21 kDa) and immunoprecipitation (p21/cyclin A/Cdk2 complexes, native and denatured). The availability of a monoclonal antibody reacting specifically with p21WAF1/Cip1 enables the subcellular detection and localization of p21WAF1/Cip1 and the measurement of relative differences in p21WAF1/Cip1 levels as a function of cell cycle phase.

Azioni biochim/fisiol

Regulation of cell cycle progression in eukaryotic cells depends on the expression of proteins called cyclins. These proteins form complexes with several different cyclin dependent kinases (CDKs). The catalytic activity of cyclin dependent kinases (CDK) is regulated by two general mechanisms: protein phosphorylation and association with regulatory subunits, which include the cyclins and the CDK inhibitors (CKIs). These inhibit all kinases involved in the G1/S transition. The the p16INK4a family, which includes p15INK4b, p16INK4a, p18INK4c and p19INK4d, inhibits Cdk4 and Cdk6 specifically.
The p21WAF1/Cip1 inhibit all kinases involved in the G1/S transition of cell cycle. p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is relatively a poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.

Stato fisico

Solution in phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Valentina Cecchinato et al.
Biochemical pharmacology, 74(11), 1568-1574 (2007-09-18)
Resveratrol (RES) is a natural occurring phytoalexin that has been shown to have chemopreventive activity. Resveratrol acts both by suppressing cell proliferation and inducing apoptosis in a variety of cancer cell lines. In this study, we show that RES induces
Bastien Vergoni et al.
Diabetes, 65(10), 3062-3074 (2016-07-09)
Activation of the p53 pathway in adipose tissue contributes to insulin resistance associated with obesity. However, the mechanisms of p53 activation and the effect on adipocyte functions are still elusive. Here we found a higher level of DNA oxidation and
Tumor susceptibility of p21Waf1/Cip1-deficient mice
Martin- Caballero J, et al.
Cancer research, 61(16), 6234-6238 (2001)
Jessica L Slack et al.
Cellular and molecular life sciences : CMLS, 68(4), 709-720 (2010-08-14)
The recent approvals of anticancer therapeutic agents targeting the histone deacetylases and DNA methyltransferases have highlighted the important role that epigenetics plays in human diseases, and suggested that the factors controlling gene expression are novel drug targets. Protein arginine deiminase
T Feng et al.
Oncogenesis, 6(1), e294-e294 (2017-01-31)
TGF-β signaling in liver cells has variant roles in the dynamics of liver diseases, including hepatocellular carcinoma (HCC). We previously found a correlation of high levels of the important endogenous negative TGF-β signaling regulator SMAD7 with better clinical outcome in

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