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MAK197

Sigma-Aldrich

Succinate Dehydrogenase Assay Kit

Sufficient for 100 Colorimetric tests

Sinonimo/i:

SDH Activity Assay Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

Metodo di rivelazione

colorimetric

Malattie correlate

neurological disorders

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... SDHA(6389) , SDHAF1(644096) , SDHAF2(54949) , SDHB(6390) , SDHC(6391) , SDHD(6392)
mouse ... SDHA(66945) , SDHAF1(68332) , SDHAF2(66072) , SDHB(67680) , SDHC(66052) , SDHD(66925)
rat ... SDHA(157074) , SDHAF2(361726) , SDHB(298596) , SDHC(289217) , SDHD(363061)

Descrizione generale

Succinate dehydrogenase (SDH; EC 1.3.5.1) is a mitochondrial enzyme that catalyzes the oxidation of succinate to fumarate and carries electrons from FADH to CoQ in eukaryotes and bacteria. It has a central function in the maintenance of cellular energy metabolism via the Krebs (tricarboxylic acid) cycle and the electron transport chain. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome.

Applicazioni

Succinate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of succinate dehydrogenase in samples.

Compatibilità

Suitable for the detection of Succinate Dehydrogenase Activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principio

The Succinate Dehydrogenase Activity Colorimetric Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

Solo come componenti del kit

N° Catalogo
Descrizione
  • SDH Assay Buffer
  • SDH Substrate Mix
  • SDH Probe
  • DCIP Standard, 2 mM
  • SDH Positive Control

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

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