MAK099
Glutamate Dehydrogenase (GDH) Activity Assay Kit
sufficient for 100 colorimetric tests
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About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prodotti consigliati
impiego
sufficient for 100 colorimetric tests
Metodo di rivelazione
colorimetric
Malattie correlate
cancer; gastrointestinal diseases
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... GLUD1(2746) , GLUD2(2747)
mouse ... GLUD1(14661)
rat ... GLUD1(24399)
Descrizione generale
Glutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas. GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH.
Applicazioni
Glutamate Dehydrogenase (GDH) Activity Assay Kit has been used to determine the enzymatic activity of glutamate dehydrogenase.
Caratteristiche e vantaggi
Compatible with high-throughput handling systems.
Compatibilità
Suitable for detecting glutamate dehydrogenase (GDH) activity in serum or tissue and cell extracts.
Principio
GDH activity is determined by a coupled enzyme assay in which glutamate is consumed by GDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the GDH activity present. One unit of GDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.6 at 37 °C.
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Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
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