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MAK004

Sigma-Aldrich

Glutamate Assay Kit

sufficient for 100 colorimetric tests

Sinonimo/i:

L-Glutamate Assay Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric tests

applicazioni

cosmetics
food and beverages

Metodo di rivelazione

colorimetric

Malattie correlate

neurological disorders; cancer

Temperatura di conservazione

−20°C

Descrizione generale

Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter. It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease. Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

Applicazioni

Glutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model. It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.

Compatibilità

Suitable to determine glutamate concentration in a variety of samples.

Principio

Glutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.

Pittogrammi

Health hazardCorrosion
GHS08,GHS05

Avvertenze

Danger

Indicazioni di pericolo

H314,H334

Classi di pericolo

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Developing medications targeting glutamatergic dysfunction in autism: progress to date.
Fung L K and Antonio Y H
CNS Drugs, 29(6), 453-463 (2015)
Glutamate as a neurotransmitter in the healthy brain.
Zhou Y and Danbolt N C
Journal of Neural Transmission. General Section, 121(8), 799-817 (2014)
Leena S Knight et al.
Neurochemical research, 42(12), 3490-3503 (2017-09-18)
Betaine (N-trimethylglycine), a common osmolyte, has received attention because of the number of clinical reports associating betaine supplementation with improved cognition, neuroprotection and exercise physiology. However, tissue analyses report little accumulation of betaine in brain tissue despite the presence of
Glutamate, at the interface between amino acid and carbohydrate metabolism.
Brosnan J T.
The Journal of Nutrition, 130(4), 988S-990S (2000)
The GluN1/GluN2B NMDA receptor and metabotropic glutamate receptor 1 negative allosteric modulator has enhanced neuroprotection in a rat subarachnoid hemorrhage model.
Zhang Z, et al.
Experimental Neurology, 301, 13-25 (2018)
Visualizza tutti i documenti correlati

Articoli

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

Protocolli

Enzymatic Glutamate Assay

Assay protocol for the colorimetric detection of Glutamate in biological samples using the Glutamate assay kit.

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