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Key Documents

M9942

Sigma-Aldrich

Monoclonal Anti-MAP2 antibody produced in mouse

clone HM-2, purified from hybridoma cell culture

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

HM-2, monoclonal

Forma fisica

buffered aqueous solution

PM

antigen ~280 kDa

Reattività contro le specie

rat, chicken, human, mouse, bovine, quail

Confezionamento

antibody small pack of 25 μL

Concentrazione

~2 mg/mL

tecniche

immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using rat brain extract

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

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Descrizione generale

MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and neurons have reached their mature morphology.
MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.

Specificità

Reacts with all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. Does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.
This antibody recognizes all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. It does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.

Immunogeno

rat brain microtubule-associated proteins (MAPs).

Applicazioni

Anti-MAP2 antibody has been used in immunofluorescence. It has also been used in immunohistochemistry.
May be used in various immunochemical techniques including immunoblotting, immunohistochemistry, and immunocytochemistry. Monoclonals antibodies to MAP2 are used to study the cytoskeleton structure in cells.
By immunoblotting, a working antibody concentration of 1-2 μg/ml is recommended using a rat brain extract.

Azioni biochim/fisiol

MAP2 (microtubule-associated protein 2) expression is monitored to predict the disease progression in glioma. MAP2 is critical in the formation of dendritic protrusions in astrocytes and oligodendrocytes. In eukaryotes, MAP2 is essential for spindle assembly and chromosome segregation.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8 C for up to one month. For extended storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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miR-484/MAP2/c-Myc-positive regulatory loop in glioma promotes tumor-initiating properties through ERK1/2 signaling.
Yi R, et al.
Journal of Molecular Histology, 49(2) (2018)
Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair
Li X, et al.
Biomaterials, 34(21), 5107-5116 (2013)
Keishi Narita et al.
PloS one, 6(1), e14575-e14575 (2011-02-02)
Glutamate excitotoxicity is one of the major events that takes place during various neurotoxic injuries such as brain ischemia. We prepared grape seed extracts, from two different varieties, containing high amounts of polyphenols but little resveratrol. Their neuroprotective effects were
Lipolysaccharide-induced neuroinflammation is associated with Alzheimer-like amyloidogenic axonal pathology and dendritic degeneration in rats.
Deng X, et al.
Advances in Alzheimer's disease, 3(2), 78-78 (2014)
On and around microtubules: an overview.
Wade R H.
Molecular Biotechnology, 43(2), 177-191 (2009)

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