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M8662

Sigma-Aldrich

Olio minerale

PCR Reagent

Sinonimo/i:

Mineral oil for PCR

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12181504
NACRES:
NA.52

Grado

PCR Reagent

Forma fisica

liquid

Confezionamento

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

tecniche

PCR: suitable

Colore

colorless

Indice di rifrazione

n20/D 1.467 (lit.)

Densità

0.84 g/mL at 25 °C (lit.)

Attività estranea

DNase, RNase, protease, none detected

Temperatura di conservazione

room temp

InChI

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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Descrizione generale

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Applicazioni

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

Caratteristiche e vantaggi

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

No data available

Punto d’infiammabilità (°C)

No data available

Dispositivi di protezione individuale

Eyeshields, Gloves


Certificati d'analisi (COA)

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Protocolli

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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