L9764
Lipopolysaccharides (rough strains) from Salmonella enterica serotype minnesota Re 595 (Re mutant)
Sinonimo/i:
LPS
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About This Item
Prodotti consigliati
Origine biologica
Salmonella enterica (Serotype minnesota Re 595 mutant)
Livello qualitativo
Stato
lyophilized powder
Impurezze
<3% Protein (Lowry)
Colore
white to yellow cast
Solubilità
water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Categorie correlate
Descrizione generale
This product is prepared from Salmonella Minnesota strain Re 595 (Re mutant) using a phenol-chloroform-petroleum ether extraction procedure. The source strain is ATCC 49284. A unique structure in the LPS of strain Re 595 was shown to induce secretion and aggregation in human platelets.
Applicazioni
- Charge and aggregation pattern govern the interaction of plasticins with LPS monolayers mimicking the external leaflet of the outer membrane of Gram-negative bacteria: This study examines how charge and aggregation patterns affect the interaction of plasticins with lipopolysaccharides (LPS) from rough strains like Salmonella enterica serotype Minnesota Re 595. The findings contribute to understanding the molecular interactions involved in microbial resistance mechanisms (Michel et al., 2015).
Azioni biochim/fisiol
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Nota sulla preparazione
Prepared by phenol-chloroform-petroleum ether extraction.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Altre note
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Oral
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Gloves
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