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Documenti fondamentali

L9267

Sigma-Aldrich

Lectin from Lens culinaris (lentil)

lyophilized powder

Sinonimo/i:

Lens culinaris agglutinin, LcH

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Forma fisica

lyophilized powder

Livello qualitativo

Potenza

<16 μg per mL agglutination activity

Impurezze

salt, free

Temperatura di conservazione

2-8°C

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Descrizione generale

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Applicazioni

Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).

Azioni biochim/fisiol

LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.

Qualità

Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.

Nota sulla preparazione

Highly purified by affinity chromatography.

Risultati analitici

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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