L8777
Lectin from Phytolacca americana (pokeweed)
lyophilized powder, BioReagent, suitable for cell culture
Sinonimo/i:
PWM, Pokeweed mitogen
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About This Item
Prodotti consigliati
Sterilità
aseptically filled
Livello qualitativo
Nome Commerciale
BioReagent
Stato
lyophilized powder
Potenza
≤0.3 μg/mL mitogenic activity
tecniche
cell culture | mammalian: suitable
Solubilità
PBS: 1 mg/mL
Temperatura di conservazione
2-8°C
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Descrizione generale
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Applicazioni
Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).
Azioni biochim/fisiol
Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Qualità
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Risultati analitici
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1 - Skin Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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