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L8902

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-M, lyophilized powder, BioReagent, suitable for cell culture

Sinonimo/i:

Phaseolus vulgaris agglutinin, PHA

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About This Item

Numero CE:
Codice UNSPSC:
12352202
NACRES:
NA.32

Livello qualitativo

Nome Commerciale

BioReagent

Forma fisica

lyophilized powder

Potenza

<40 μg per mL agglutination activity

tecniche

cell culture | mammalian: suitable

Impurezze

salt, free

Solubilità

PBS: 2 mg/mL

Temperatura di conservazione

2-8°C

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Categorie correlate

Applicazioni

Lectin from Phaseolus vulgaris (red kidney bean) is used in Lymphocyte Transformation Assay and culture medium.

Azioni biochim/fisiol

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Risultati analitici

Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C.

Prodotti correlati

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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