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Key Documents

L1668

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Sinonimo/i:

Phaseolus vulgaris agglutinin, PHA

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Livello qualitativo

200

Nome Commerciale

BioReagent

Forma fisica

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

Potenza

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

tecniche

cell culture | mammalian: suitable

Colore

white to off-white

Solubilità

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

Temperatura di conservazione

2-8°C

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Categorie correlate

Applicazioni

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for the stimulation of cluster of differentiation (CD4+) T cells
  • for the activation of primary peripheral blood lymphocytes
  • for the stimulation of peripheral blood mononuclear cells

Azioni biochim/fisiol

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Confezionamento

Sold on the basis of lectin content

Risultati analitici

Potency and solubility expressed in mg lectin/ml

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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