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L2879

Sigma-Aldrich

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

Sinonimo/i:

Peptidoglycan iV-acetylmuramoyl hydrolase, Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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About This Item

Numero CAS:
9066-59-5
Classificazione EC (Enzyme Commission):
3.2.1.17 (BRENDA, IUBMB)
Numero CE:
232-954-0
Numero MDL:
MFCD00131557
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

chicken egg white

Livello qualitativo

200

Tipo

Grade VI

Forma fisica

lyophilized powder

Attività specifica

≥35,000 units/mg protein (E1%/282)

PM

~14.3 kDa

Composizione

Protein, ≥80%

tecniche

cell based assay: suitable

Compatibilità

suitable for cell lysis

applicazioni

cell analysis

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Lysozyme, also known as muramidase, is a bacteriolytic enzyme found in phagocytic cells like leukocytes. The name "lysozyme" is derived from its ability to dissolve or hydrolyze the β-1-4-glycosidic bond between N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) in the walls of certain bacteria. This protein is present in animals, plants, bacteria, and viruses. It can also be found in granules of neutrophils, macrophages, as well as in serum, saliva, milk, honey, and hen egg white. There are different types of lysozymes, including the c-type (chicken type), the g-type (goose-type), and the i-type (invertebrates).

Applicazioni

Lysozyme chloride form from chicken egg white has been used:
  • as a component in the protein mixture to study the effects of yeast cells on membrane fouling caused by a protein mixture in dead-end filtration
  • in dip system to study its bacteriostatic or bactericidal effects on Salmonella-contaminated broiler parts
  • in viscometric assay for determining lysozyme activity    

Azioni biochim/fisiol

Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria.

Definizione di unità

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Stato fisico

Lyophilized powder containing sodium chloride and sodium acetate

Nota sulla preparazione

3× Crystallized

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Journal of analytical toxicology, 32(4), 281-291 (2008-04-24)
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A Sobrino-López et al.
Journal of dairy science, 91(5), 1759-1768 (2008-04-19)
High-intensity pulsed electric field (HIPEF) is a nonthermal treatment studied for its wide antimicrobial spectrum on liquid food, including milk and dairy products. Moreover, the antimicrobial effect of HIPEF may be enhanced by combining HIPEF with other treatments as hurdles.
Zhe Wang et al.
The journal of physical chemistry. B, 117(4), 1186-1195 (2013-01-11)
High-resolution inelastic X-ray scattering was used to investigate the collective vibrational excitations in hydrated lysozyme powders as a function of hydration level and temperature. It is found that the samples with strong enzymatic function are "soft", in the sense that
Sven O Dahms et al.
Acta crystallographica. Section D, Biological crystallography, 69(Pt 2), 284-297 (2013-02-07)
Heavy-atom clusters (HA clusters) containing a large number of specifically arranged electron-dense scatterers are especially useful for experimental phase determination of large complex structures, weakly diffracting crystals or structures with large unit cells. Often, the determination of the exact orientation
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Articoli

Carbohydrate Analysis

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Protocolli

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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