Passa al contenuto
Merck
Tutte le immagini(2)

Documenti fondamentali

L1667

Sigma-Aldrich

Lysozyme human

Lysobac, recombinant, expressed in rice, ≥100,000 units/mg protein, lyophilized powder

Sinonimo/i:

Lysobac r Lysozyme, Mucopeptide N-acetylmuramoylhydrolase, Muramidase

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

human

Livello qualitativo

Ricombinante

expressed in rice

Saggio

≥90% (SDS-PAGE)

Stato

lyophilized powder

Attività specifica

≥100,000 units/mg protein

PM

16.5 kDa

tecniche

cell based assay: suitable

Compatibilità

suitable for cell lysis

N° accesso UniProt

applicazioni

cell analysis

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... LYZ(4069)

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein. Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa. It is a positively charged and a C-type lysozyme with α and β structural domains.

Applicazioni

Lysozyme human has been used:
  • in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques
  • in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system
  • in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)

Azioni biochim/fisiol

Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells. It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality. In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue. Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.

Caratteristiche e vantaggi

Lysobac is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:
  • Improves Bacterial Fermentation Efficiency
  • Greater bacterial lysis activity per mg.
  • 1 gram of delivers more lysis activity than 4 grams of HEWL
  • Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
  • Improved recombinant protein yield compared to mechanical lysis
  • Reduces cost of goods

Confezionamento

Package size based on protein content

Definizione di unità

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Note legali

Lysobac is a trademark of InVitria

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 8

1 of 8

Lisozima 10 mg/mL

Sigma-Aldrich

L3790

Lisozima

Lisozima For use as a marker in SDS-PAGE

Sigma-Aldrich

L4631

Lisozima

Lysozyme (from egg white) ≥ 30 000 FIP-U/mg cryst. (hydrochloride) for biochemistry EC 3.2.1.17

SAFC

1.05281

Lysozyme

Lisozima VETRANAL®, analytical standard

Supelco

34046

Lisozima

Thermodynamics and binding mechanism of polyphenon-60 with human lysozyme elucidated by calorimetric and spectroscopic techniques
Yasmeen S, et al.
The Journal of Chemical Thermodynamics, 110(5), 79-86 (2017)
A novel lysozyme mutation Phe57Ile associated with hereditary renal amyloidosis
Yazaki M, et al.
Kidney International, 63(5), 1652-1657 (2003)
Suppression of human arthritis synovial fibroblasts inflammation using dexamethasone-carbon nanotubes via increasing caveolin-dependent endocytosis and recovering mitochondrial membrane potential
Lee YK, et al.
International journal of nanomedicine, 12(5), 5761-5761 (2017)
Inflammatory bowel disease: an impaired barrier disease
Jager S, et al.
Langenbeck'S Archives of Surgery / Deutsche Gesellschaft Fur Chirurgie, 398(1), 1-12 (2013)
Expression of natural antimicrobial human lysozyme in rice grains
Huang J, et al.
Molecular breeding : new strategies in plant improvement, 10(1-2), 83-94 (2002)

Articoli

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Protocolli

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.