L2380
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
lyophilized powder
Sinonimo/i:
Bandeirea simplicifolia agglutinin, BS-I
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
Potenza
<20 μg per mL agglutination activity (using human blood group B erythrocytes)
Composizione
Protein, ≥90% biuret
Impurezze
salt, free
Temperatura di conservazione
2-8°C
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Azioni biochim/fisiol
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Altre note
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Nota sulla preparazione
Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).
Risultati analitici
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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