GE17-1151-01
HiTrap® Sp High Performance
Cytiva 17-1151-01, pack of 5 × 1 mL
About This Item
Prodotti consigliati
ligand
sulphopropyl
Descrizione
Ion Exchanger Type (value)
Confezionamento
pack of 5 × 1 mL
Produttore/marchio commerciale
Cytiva 17-1151-01
Parametri
flow rate
42 psi
Dimensioni del letto
7 mm × 25 mm
Volume del letto
1 mL
Diam. int. colonna
7 mm
Matrice
6% cross-linked agarose
Dimensione particelle
24-44 μm
Dimens. media delle particelle
34 μm
cleaning
3-14
Intervallo di esercizio
4-13
Compatibilità
suitable for bioprocess medium
Descrizione generale
The small particle size (34μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose™ High Performance is a strong cation exchange medium and Q Sepharose™ High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.
Caratteristiche e vantaggi
- 34 μm bead size for high-performance, high-resolution purifications.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
- Strong sulfopropyl (SP) cation exchanger.
Stoccaggio e stabilità
Risultati analitici
Note legali
Prodotti correlati
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
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Articoli
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
Protocolli
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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