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Key Documents

GE17-1153-01

HiTrap® Q High Performance

Cytiva 17-1153-01, pack of 5 × 1 mL

Sinonimo/i:

Q-Sepharose High Performance

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

quaternary amine

Descrizione

Ion Exchanger Type (value)

Confezionamento

pack of 5 × 1 mL

Produttore/marchio commerciale

Cytiva 17-1153-01

Parametri

<4 mL/min flow rate
42 psi

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Matrice

6% cross-linked agarose

Dimensione particelle

24-44 μm

Dimens. media delle particelle

34 μm

cleaning

1-14

working range

2-12

Compatibilità

suitable for bioprocess medium

Descrizione generale

HiTrap® Q HP are prepacked, ready-to-use Q Sepharose High Performance strong anion exchange columns for high-resolution, small-scale protein purification.

Applicazioni

Techniques & Applications: Separation of protein using a strong anion exchange medium prepacked in the HiTrap® column format.

Caratteristiche e vantaggi

  • 34 μm bead size for high-performance, high-resolution purifications.
  • Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
  • Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
  • Strong quaternary ammonium (Q) anion exchanger

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

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Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Vedi tutto

Protocolli

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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