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Key Documents

GE17-1408-01

HiTrap® Desalting Columns

Cytiva 17-1408-01, pack of 5 × 5 mL

Sinonimo/i:

Purification Columns

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

Confezionamento

pack of 5 × 5 mL

Produttore/marchio commerciale

Cytiva 17-1408-01

Condizioni di stoccaggio

dry media

Parametri

42 psi

Dimensioni del letto

16 mm × 25 mm

Volume del letto

5 mL

Diam. int. colonna

16 mm

Matrice

cross-linked dextran

Dimensione particelle

>20 μm (dry)

cleaning

2-13

working range

2-13

Compatibilità

suitable for bioprocess medium

Categorie correlate

Descrizione generale

HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange.

HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

Applicazioni

Desalting and buffer exchange with Sephadex® G-25.

Caratteristiche e vantaggi

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Vedi tutto

Protocolli

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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