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Key Documents

GE17-5087-02

Sigma-Aldrich

HiPrep 26/10 Desalting

Cytiva 17-5087-02, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Sinonimo/i:

Desalting Column, HiPrep Desalting Column

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

Materiali

polypropylene (PP)

PM

1000-5000 Da (globular proteins)

Confezionamento

pack of 4

Produttore/marchio commerciale

Cytiva 17-5087-02

Condizioni di stoccaggio

dry media

Parametri

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

Volume del letto

53 mL

Lungh. × diam. int. colonna

10 cm × 26 mm

Matrice

cross-linked dextran
polymeric

Gruppo funzionale matrice

dextran-epichlorohydrin phase

Dimensione particelle

17-132 μm (dry)

cleaning

2-13

working range

2-13

Tecnica di separazione

size exclusion (SEC)

Descrizione generale

HiPrep 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Caratteristiche e vantaggi

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Compatibilità

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Vedi tutto

Protocolli

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

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