GE17-0981-01
CNBr Activated Sepharose™ 4 Fast Flow
Cytiva 17-0981-01, pack of 10 g
Sinonimo/i:
Affinity chromatography resin
About This Item
Prodotti consigliati
ligand
cyanogen bromide
Confezionamento
pack of 10 g
Produttore/marchio commerciale
Cytiva 17-0981-01
Condizioni di stoccaggio
(Unsused: 2 - 8°C - Used: 2 - 8°C in 20% Ethanol)
Matrice
4% cross-linked agarose
Dimensione particelle
45-165 μm
Diametro medio
90 μm
cleaning in place
2-11(Ligand dependent)
working range
3-11(ligand dependent)
Compatibilità
suitable for bioprocess medium
Temperatura di conservazione
2-8°C
Descrizione generale
CNBr-activated Sepharose™ 4 Fast Flow is based on the well established Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide. CNBr-activated Speharose 4 Fast Flow is designed for multipoint attachment of protein ligands containing amino groups.
The preparation and use of affinity chromatography media by coupling biospecific ligands to CNBr-activated Sepharose™ 4 Fast Flow is a widely used, successful and well-documented approach, with an easy, rapid and efficient coupling procedure.
CNBr-activated Sepharose™ 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, CNBr-activated Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Caratteristiche e vantaggi
- CNBr-activated BioProcess medium designed for coupling of large amino-containing ligands.
- Easy, rapid and efficient coupling
- This resin allows for multi-point attachment of protein ligands which minimizes ligand leakage
- BioProcess medium supported for industrial applications and well-established in approved processes
Stoccaggio e stabilità
Risultati analitici
Note legali
Codice della classe di stoccaggio
13 - Non Combustible Solids
Certificati d'analisi (COA)
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Protocolli
This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.
While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
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