G8511
β-Galactosidase from Escherichia coli
For use as a marker in SDS-PAGE
Sinonimo/i:
β-D-Galactoside galactohydrolase, Lactase
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About This Item
Prodotti consigliati
PM
~116 kDa
Confezionamento
vial of 0.5 mg
Temperatura di conservazione
2-8°C
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Applicazioni
β-Galactosidase from Escherichia coli has been used as an internal standard in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It has been used in the protein ladder mix for optimization of microfluidic western blotting assay.
Azioni biochim/fisiol
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Proprietà fisiche
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Definizione di unità
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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