P1261
Phosphorylase a from rabbit muscle
lyophilized powder, 20-30 units/mg protein
Sinonimo/i:
1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase
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About This Item
Prodotti consigliati
Origine biologica
rabbit muscle
Livello qualitativo
Stato
lyophilized powder
Attività specifica
20-30 units/mg protein
Purificato mediante
2× crystallization
Composizione
Protein, 30-60% E1%/280
Attività estranea
AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
phosphoglucomutase ≤0.25%
phosphorylase b ≤30%
Temperatura di conservazione
−20°C
Descrizione generale
Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).
Applicazioni
Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.
Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP.
Azioni biochim/fisiol
Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls.
Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.
Definizione di unità
One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)
Stato fisico
Lyophilized powder containing β-glycerophosphate and EDTA
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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