G2500
Gelatina
gel strength 300, Type A
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About This Item
Prodotti consigliati
Origine biologica
Porcine skin
Livello qualitativo
Tipo
Type A
Saggio
70-90% (biuret)
Forma fisica
powder
tecniche
cell culture | mammalian: suitable
Resistenza del gel
~300 g Bloom
Solubilità
H2O: soluble 50 mg/mL
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Descrizione generale
Gelatin is a hydrocolloid. Bovine or porcine bone and hide are its commercial sources. It is a collagen derivative and comprises protein as a major component with minerals and water.
Applicazioni
Gelatin from porcine skin has been used:
- for coating glass-bottomed plates for culturing A375 cells and in gelatin degradation assay
- for coating glass slides in the histological studies with brain sections
- in the synthesis of gelatin methacrylate (GelMA) prepolymer for encapsulating cells on to 3D printed hydrogels
- to prepare thiol-functionalized gelatin (GelSH) hydrogel for 3D projection bioprinting
Gelatin from porcine skin is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Azioni biochim/fisiol
Gelatin polymer is an alternative biomaterial to collagen for cell and tissue culture. It is a much sorted gelling agent useful as a thickener and food ingredient. Gelatin is also used as a foaming and binding agent and as well as in the pharmaceutical industry. It is biodegradable and biocompatible with biomedical applications especially in biomaterials for cardiac and vascular tissue regeneration.
Componenti
La gelatina è una miscela eterogenea di proteine solubili in acqua ad alta massa molecolare media, presente nel collagene. Le proteine si estraggono facendo bollire in acqua pelle, tendini, legamenti, ossa, ecc. adatti. La gelatina di tipo A deriva da tessuto trattato con acidi. Quella di tipo B da tessuto trattato con calce.
Avvertenza
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
Nota sulla preparazione
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
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Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
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