E8656

Heat-Labile Enterotoxin, B subunit

recombinant, expressed in Pichia pastoris, >90% (SDS-PAGE), lyophilized powder

• Sinonimo/i: LTB
• Codice UNSPSC: 12352200
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in Pichia pastoris
• Livello qualitativo: 200
• Saggio: >90% (SDS-PAGE)
• Stato: lyophilized powder
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Applicazioni

Heat-Labile Enterotoxin, B subunit (LTB) from E. coli has been used in enzyme-linked immunosorbent assay (ELISA).

Azioni biochim/fisiol

Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside G_M1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside G_M1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM₁ on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant.

Caratteristiche e vantaggi

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Stato fisico

Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃, and 1 mM sodium EDTA.

Risultati analitici

The activity is measured by ELISA using ganglioside G_M1-coated plates (Cat. No. G7641). LTB at various concentrations is incubated on the ganglioside G_M1 coated wells, followed by anti-cholera toxin antibody (Cat. No. C3062), and peroxidase-labeled goat anti-rabbit IgG (Cat.No. A0545) as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H312,H412
• Consigli di prudenza: P273 - P280 - P302 + P352 + P312 - P362 + P364 - P501
• Classi di pericolo: Acute Tox. 4 Dermal - Aquatic Chronic 3
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter