P9582
Polyinosinic–polycytidylic acid potassium salt
with buffer salts, TLR ligand tested
Sinonimo/i:
Poly (I:C), Poly(I) • Poly(C)
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About This Item
Prodotti consigliati
Livello qualitativo
Saggio
≥99% (less than 1% free nucleotides, TLC)
Forma fisica
lyophilized powder
Composizione
Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)
Condizioni di stoccaggio
desiccated
Temperatura di conservazione
−20°C
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Applicazioni
TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.
Azioni biochim/fisiol
Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.
Confezionamento
Package size based on polynucleotide content
Altre note
Double-stranded homopolymer.
Nota sulla preparazione
The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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