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Key Documents

Altri documenti

E1388

Sigma-Aldrich

β-Endothelial Cell Growth Factor human

β-ECGF, recombinant, expressed in E. coli, suitable for cell culture

Sinonimo/i:

ECGS, β-ECGF

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About This Item

Numero CAS:
105843-51-4
Numero MDL:
MFCD00131012
Codice UNSPSC:
12352202
NACRES:
NA.77

Origine biologica

human

Livello qualitativo

200

Ricombinante

expressed in E. coli

Saggio

≥97% (SDS-PAGE)

Forma fisica

lyophilized powder

Potenza

0.03-0.3 ng/mL ED50/EC50

Qualità

endotoxin tested

PM

predicted mol wt ~17 kDa

Confezionamento

pkg of 25 μg

Condizioni di stoccaggio

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

tecniche

cell culture | mammalian: suitable

Impurezze

≤1.0 EU/μg protein Endotoxin level (LAL test)

N° accesso UniProt

P49765

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... VEGFB(7423)

Categorie correlate

Descrizione generale

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

Applicazioni

β-Endothelial Cell Growth Factor human has been used:
  • in cell death assay
  • in endothelial cell culture
  • in human umbilical vascular endothelial cell (HUVEC) culture

Azioni biochim/fisiol

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Altre note

14 amino acid, N-terminal extended form of human FGF-acidic.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Risultati analitici

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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A blood pressure-associated variant of the SLC39A8 gene influences cellular cadmium accumulation and toxicity
Zhang R, et al.
Human Molecular Genetics, 25(18), 4117-4126 (2016)
Serum vascular endothelial growth factor B is elevated in women with polycystic ovary syndrome and can be decreased with metformin treatment
Cheng F, et al.
Clinical Endocrinology, 84(3), 386-393 (2016)
Eleanor G Lynam et al.
Methods in molecular biology (Clifton, N.J.), 2475, 223-228 (2022-04-23)
Endothelial cell proliferation rate is an important indicator of vascular health. Being able to detect the rate of endothelial cell proliferation, or cell cycle disturbances after intervention is a valuable tool for analysing any beneficial or detrimental effects of treatments
Chuan-Rong Zhao et al.
Small (Weinheim an der Bergstrasse, Germany), 19(2), e2204694-e2204694 (2022-11-21)
Disturbed blood flow induces endothelial pro-inflammatory responses that promote atherogenesis. Nanoparticle-based therapeutics aimed at treating endothelial inflammation in vasculature where disturbed flow occurs may provide a promising avenue to prevent atherosclerosis. By using a vertical-step flow apparatus and a microfluidic
Bioinformatics analyses of the role of vascular endothelial growth factor in patients with non-small cell lung cancer
Wang Y, et al.
PLoS ONE, 10(9), e0139285-e0139285 (2015)
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