E1388
β-Endothelial Cell Growth Factor human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sinonimo/i:
ECGS, β-ECGF
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Nome del prodotto
β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
Origine biologica
human
Livello qualitativo
Ricombinante
expressed in E. coli
Saggio
≥97% (SDS-PAGE)
Stato
lyophilized powder
Potenza
0.03-0.3 ng/mL ED50/EC50
Qualità
endotoxin tested
PM
predicted mol wt ~17 kDa
Confezionamento
pkg of 25 μg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
tecniche
cell culture | mammalian: suitable
Impurezze
≤1.0 EU/μg protein Endotoxin level (LAL test)
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... VEGFB(7423)
Descrizione generale
The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.
Applicazioni
β-Endothelial Cell Growth Factor human has been used:
- in cell death assay
- in endothelial cell culture
- in human umbilical vascular endothelial cell (HUVEC) culture
Azioni biochim/fisiol
β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.
Altre note
14 amino acid, N-terminal extended form of human FGF-acidic.
Stato fisico
Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.
Risultati analitici
The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
A blood pressure-associated variant of the SLC39A8 gene influences cellular cadmium accumulation and toxicity
Zhang R, et al.
Human Molecular Genetics, 25(18), 4117-4126 (2016)
Serum vascular endothelial growth factor B is elevated in women with polycystic ovary syndrome and can be decreased with metformin treatment
Cheng F, et al.
Clinical Endocrinology, 84(3), 386-393 (2016)
Eleanor G Lynam et al.
Methods in molecular biology (Clifton, N.J.), 2475, 223-228 (2022-04-23)
Endothelial cell proliferation rate is an important indicator of vascular health. Being able to detect the rate of endothelial cell proliferation, or cell cycle disturbances after intervention is a valuable tool for analysing any beneficial or detrimental effects of treatments
Chuan-Rong Zhao et al.
Small (Weinheim an der Bergstrasse, Germany), 19(2), e2204694-e2204694 (2022-11-21)
Disturbed blood flow induces endothelial pro-inflammatory responses that promote atherogenesis. Nanoparticle-based therapeutics aimed at treating endothelial inflammation in vasculature where disturbed flow occurs may provide a promising avenue to prevent atherosclerosis. By using a vertical-step flow apparatus and a microfluidic
Bioinformatics analyses of the role of vascular endothelial growth factor in patients with non-small cell lung cancer
Wang Y, et al.
PLoS ONE, 10(9), e0139285-e0139285 (2015)
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.