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D9515

Sigma-Aldrich

Driselase from Basidiomycetes sp.

greener alternative

powder, Protein ≥10 % by biuret

Sinonimo/i:

cellulase, laminarinase, xylanase

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Coniugato

conjugate (Glucosaminoglycan)

Livello qualitativo

Stato

powder

Composizione

Protein, ≥10% biuret

Caratteristiche più verdi

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Categoria alternativa più verde

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

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Descrizione generale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Applicazioni

Driselase from Basidiomycetes has been used in a study to assess the digestion by fungal glycanases of arabinoxylans with different feruloylated side-chains. Driselase from Basidiomycetes has also been used in a study to investigate the purification, characterization, and mode of action of a rhamnogalacturonan hydrolase.
The enzyme from Sigma has been used as a control while testing the ability of p-coumaroyl esterase to release p-coumaroyl and feruloyl groups from intact cell walls. It has also been used in the protoplast preparation from mycelia during a study to investigate the pathogenicity of Cochliobolus carbonum on Maize.

Azioni biochim/fisiol

Driselase is a cell wall degrading enzyme that contains cellulase, hemicellulase, pectinase etc. Therefore, it is very effective in removing plant cell walls to make protoplasts.

Altre note

Crude powder containing laminarinase, xylanase and cellulase.

Note legali

Driselase is a trademark of ASKA Animal Health Co. Ltd.

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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W S Borneman et al.
Applied and environmental microbiology, 57(8), 2337-2344 (1991-08-01)
An extracellular p-coumaroyl esterase produced by the anaerobic fungus Neocallimastix strain MC-2 released p-coumaroyl groups from 0-[5-0-((E)-p-coumaroyl)-alpha-L-arabinofuranosyl]-(1----3)-0-beta -D-xylopyranosyl-(1----4)-D-xylopyranose (PAXX). The esterase was purified 121-fold from culture medium in successive steps involving ultrafiltration column chromatography on S-sepharose and hydroxylapatite, isoelectric focusing
J S Scott-Craig et al.
The Plant cell, 2(12), 1191-1200 (1990-12-01)
A gene (PGN1) encoding extracellular endopolygalacturonase was isolated from the fungal maize pathogen Cochliobolus carbonum race 1. A probe was synthesized by polymerase chain reaction using oligonucleotides based on the endopolygalacturonase amino acid sequence. Genomic and cDNA copies of the
Taras Pasternak et al.
Plant methods, 11, 50-50 (2015-10-31)
Rapid advances in microscopy have boosted research on cell biology. However sample preparation enabling excellent reproducible tissue preservation and cell labeling for in depth microscopic analysis of inner cell layers, tissues and organs still represents a major challenge for immunolocalization
Q Chen et al.
Plant physiology, 94(4), 1820-1829 (1990-12-01)
Treating carrot (Daucus carota L.) suspension culture cells with a mixture of cell wall degrading enzymes, Driselase, resulted in an increase in the percentage of [(3)H]phosphatidylinositol bisphosphate. Analysis of the lipid kinase activities in the isolated plasma membranes after whole
G Wende et al.
Phytochemistry, 45(6), 1123-1129 (1997-07-01)
Alcohol-insoluble residues (AIRs) from Festuca and Zea cell cultures contained 7.4 and 35 nmol esterified ferulate mg-1, respectively. Driselase solubilised 79% of the feruloylated material from both AIRs. Of the feruloyl esters solubilised from Festuca and Zea AIRs, 72 and

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