D4941

Anti-phospho-DAP-Kinase (pSer³⁰⁸) antibody, Mouse monoclonal

clone DKPS308, purified from hybridoma cell culture

• Sinonimo/i: Anti-DAPK, Anti-ROCO3
• Numero MDL: MFCD04040944
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: DKPS308, monoclonal
• Stato: buffered aqueous solution
• PM: antigen ~160 kDa
• Reattività contro le specie: human
• tecniche: immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using 293T (human embryonal kidney) cells transfected with DAP-kinase expression vector.
• Isotipo: IgG1
• N° accesso UniProt: P53355
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pSer308)
• Informazioni sul gene: human ... DAPK1(1612)

Descrizione

Descrizione generale

Monoclonal Anti-phospho DAP-Kinase (pSer308) (mouse IgG1 isotype) is derived from the DKPS308 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphopeptide of human DAP-kinase, conjugated to KLH. Death Associated Protein Kinase (DAPK) is characterized with a multidomain structure including subdomain typical of serine/threonine kinases, a Ca²⁺/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. In addition, it also contains two auto-inhibitory domains, one of them Ca²⁺/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active.

Immunogeno

Phosphopeptide corresponding to amino acids 303-312 (pSer³⁰⁸) of human DAP-kinase, conjugated to KLH.

Applicazioni

Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal has been used in:

• western blot assay
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

Monoclonal anti-phospho-DAP-kinase (pSer³⁰⁸) antibody can be used in indirect ELISA, immunoblotting and immunoprecipitation. It can also be used in western blotting.

Azioni biochim/fisiol

Death Associated Protein Kinase (DAPK) plays crucial role in programmed cell death as well as in autophagy. DAPK activity is regulated by phosphorylation. Autophosphorylation at Ser³⁰⁸ on the calmodulin regulatory domain negatively regulate DAPK activity. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. Consequently, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used to study the mechanism of DAPK activation in apoptosis. It can also be used in microarray.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable