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D3035

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, female

Sinonimo/i:

DNA

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About This Item

Numero CAS:
9007-49-2
Numero MDL:
MFCD00130921
Codice UNSPSC:
41106310
NACRES:
NA.52

Origine biologica

human placenta

Livello qualitativo

200

Grado

for molecular biology

Forma fisica

buffered aqueous solution

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)
AWBASQCACWFTGD-UHFFFAOYSA-N

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Descrizione generale

Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.

Applicazioni

Deoxyribonucleic acid from human placenta has been used:
  • to compare the efficiency of DNA quantification methods
  • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines
  • as an internal control to estimate the degree of fragmentation of circulating DNA
  • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11
  • to compare the 70-bp P5 exon sequence between DNA of different human and primate species
  • to calculate copy number of genes, relative to normal human tissue
  • in PCR reactions
For use in Southern hybridizations.

Prodotti consigliati

Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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J E Collins et al.
Genome research, 7(5), 522-531 (1997-05-01)
A clone map consisting of YACs, cosmids, and fosmids has been constructed covering low copy repeat regions of human chromosome 22q11. A combination of clone restriction digest analysis, single-copy landmark content analysis, HindIII-Sau3AI fingerprinting, and sequencing of PCR products derived
Alain R Thierry et al.
Nucleic acids research, 38(18), 6159-6175 (2010-05-25)
Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an
Heather Hoover et al.
Journal of proteome research, 14(9), 3670-3679 (2015-07-08)
Tumor types can be defined cytologically by their regions of chromosomal amplification, which often results in the high expression of both mRNA and proteins of certain genes contained within the amplicon. An important strategy for defining therapeutically relevant targets in
Karsten Nielsen et al.
Forensic science international. Genetics, 2(3), 226-230 (2008-12-17)
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than
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