KELLY Cell Line human

92110411, human brain, Neuronal

• Sinonimo/i: NB19 Cells, NB19-RIKEN Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: KELLY Cell Line human, 92110411, neuroblastoma
• Origine biologica: human brain
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Neuronal
• Prodotti: N-myc RNA or protein
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human neuroblastoma

Descrizione della linea cellulare

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Applicazioni

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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