KELLY Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92110411, human brain, Neuronal

• Sinonimo/i: NB19 Cells, NB19-RIKEN Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human brain
• Confezionamento: tube of 5 μg 92110411-DNA-5UG; pkg of vial of cells 92110411-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Neuronal
• Prodotti: N-myc RNA or protein
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human neuroblastoma

Descrizione della linea cellulare

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Applicazioni

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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