NB4 1A3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse nerve
• Confezionamento: tube of 5 μg 89121405-DNA-5UG; pkg of vial of cells 89121405-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Unstable
• Morfologia: Neuroblast
• Prodotti: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C-1300 Neuroblastoma

Descrizione della linea cellulare

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Applicazioni

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Terreno di coltura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Altre note

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