AtT20

87021902, mouse brain, Small round cells growing as large aggregates

• Codice UNSPSC: 41106514

Proprietà

• product name: AtT20, 87021902
• Origine biologica: mouse brain
• Modalità di accrescimento: Aggregates in suspension
• Cariotipo: Diploid
• Morfologia: Small round cells growing as large aggregates
• Prodotti: Adrenocorticotropic hormone (ACTH)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse LAF1 pituitary gland tumour

Descrizione della linea cellulare

Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.

Applicazioni

Hormone studies

Terreno di coltura

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.

Mantenimento delle subcolture

Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi

Altre note

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