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Documenti fondamentali

C9284

Sigma-Aldrich

Catalase−Agarose

pH 5.4(isoelectric point), ammonium sulfate suspension, enzyme from bovine liver

Sinonimo/i:

H2O2:H2O2 Oxidoreductase

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About This Item

Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

enzyme from bovine liver

Descrizione

optimum pH ~ 7.0

Stato

ammonium sulfate suspension

PM

tetramer ~250 kDa

Concentrazione

≥800 units/mL

Grado di funzionalizzazione

60,000-120,000 units per g agarose (1900-3800 units per ml of agarose)

Matrice

4% cross-linked beaded agarose

pH

5.4(isoelectric point)

Temperatura di conservazione

2-8°C

Applicazioni

Catalase agarose has been used in a study to advance the treatment of oxidative stress in neurodegenerative diseases. Catalase agarose has also been used in a study to investigate the growth of cyanobacterium on agar media in the presence of heterotrophic bacteria.
Catalases have a wide range of industrial applications. The enzyme is used for the removal of traces of (H2O2) that is added to milk and cheese during sterilization. It is used for the degradation of peroxide used in textile bleaching to prevent any problems in subsequent dyeing. Coupled with oxidases, the enzyme is used to prevent the inactivation of oxidases by the action of high concentration of peroxide. It is also used to prevent side reactions caused by (H2O2) that can degrade the target product.

Azioni biochim/fisiol

Catalase activity is constant over the pH range of 4.0-8.5. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.

Avvertenza

Freezing of solutions is not recommended.

Definizione di unità

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Stato fisico

Suspension in 3.2 M (NH4)2SO4, pH 7, containing preservative

Inibitore

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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