C3556

Catalase from human erythrocytes

≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein

• Sinonimo/i: H₂O₂:H₂O₂ oxidoreductase
• Numero CAS: 9001-05-2
• Classificazione EC (Enzyme Commission): 1.11.1.6 (BRENDA, IUBMB)
• Numero CE: 232-577-1
• Numero MDL: MFCD00081483
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: human erythrocytes
• Livello qualitativo: 200
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: buffered aqueous solution
• Attività specifica: ≥30,000 units/mg protein
• PM: tetramer ~250 kDa
• Concentrazione: ≤10 mg/mL
• tecniche: cell based assay: suitable
• N° accesso UniProt: P04040
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3; NUVBSKCKDOMJSU-UHFFFAOYSA-N
• Informazioni sul gene: human ... CAT(847)

Descrizione

Descrizione generale

Research area: Cell Signaling

Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13.

Applicazioni

Catalase from human erythrocytes has been used:

• to prevent reoxidation of reduced cytochrome c by H2O2 while measuring the production of superoxide with cytochrome C.
• as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of human skin fibroblasts
• as a component of the gloxy mix for single-molecule imaging

Azioni biochim/fisiol

Deficiency of catalase and oxidative stress are associated with vitiligo, hypertension, Alzheimer′s disease, diabetes mellitus, and acatalasemia. Catalase shows a dichotomous role in cancer as its expression is at a higher level in chronic lymphocytic leukemia, glioma, melanoma, and gastric cancer and lower levels of expression are seen in acute myeloid leukemia, pancreatic, lung, prostate, and skin (non-melanoma) cancers.

Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis.

Definizione di unità

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Stato fisico

Solution in 50 mM Tris, pH 8.0

Stoccaggio e stabilità

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Risultati analitici

Protein determined by biuret

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)