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Key Documents

C9205

Sigma-Aldrich

Monoclonale Anti-proteina acida gliale fibrillare (GFAP)

clone G-A-5, purified from hybridoma cell culture

Sinonimo/i:

Anti-GFAP

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

CY3 conjugate

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

G-A-5, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

human, pig, rat

tecniche

direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections

Isotipo

IgG1

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... GFAP(2670)
rat ... Gfap(24387)

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Descrizione generale

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Specificità

L′anticorpo reagisce specificamente con GFAP in prove di immunoblotting e marca astrociti, cellule gliali di Bergmann e condrociti di cartilagine elastica in colorazioni immunoistochimiche. L′anticorpo reagisce con antigeni specifici delle cellule glia in sezioni di tessuto congelate o fissate in alcol.
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Immunogeno

GFAP da midollo spinale porcino
GFAP from pig spinal cord

Applicazioni

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in
  • immunohistochemistry
  • immunoblotting
  • immunolabelling,
  • immunocytochemistry
  • immunofluorescence

Azioni biochim/fisiol

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Note legali

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anticorpo

Prodotti correlati

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificati d'analisi (COA)

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D B Pettigrew et al.
BMC neuroscience, 2, 9-9 (2001-06-12)
Brain and spinal cord white matter can support extensive axonal growth. This growth is generally constrained to an orientation that is parallel to the longitudinal axis of the fiber tract. This constraint is presumably due to permissive and non-permissive substrates
Sequel of spontaneous seizures after kainic acid-induced status epilepticus and associated neuropathological changes in the subiculum and entorhinal cortex
Drexel M, et al.
Neuropharmacology, 63(5), 806-817 (2012)
Aixa F Rivera-Pagán et al.
PloS one, 10(4), e0125195-e0125195 (2015-04-18)
Excitotoxicity due to glutamate receptor over-activation is one of the key mediators of neuronal death after an ischemic insult. Therefore, a major function of astrocytes is to maintain low extracellular levels of glutamate. The ability of astrocytic glutamate transporters to
Fibroblasts can express glial fibrillary acidic protein (GFAP) in vivo
Hainfellner JA, et al.
Journal of Neuropathology and Experimental Neurology, 60(5), 449-461 (2001)
Expression of vimentin and glial fibrillary acidic protein in central nervous system development of rats
Luo H, et al.
Asian Pacific Journal of Tropical Medicine, 10(12), 1185-1189 (2017)

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