C9205

Monoclonale Anti-proteina acida gliale fibrillare (GFAP)

clone G-A-5, purified from hybridoma cell culture

• Sinonimo/i: Anti-GFAP
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Coniugato: CY3 conjugate
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: G-A-5, monoclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: human, pig, rat
• tecniche: direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections
• Isotipo: IgG1
• N° accesso UniProt: P14136
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... GFAP(2670) ; rat ... Gfap(24387)

Descrizione

Descrizione generale

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Specificità

L′anticorpo reagisce specificamente con GFAP in prove di immunoblotting e marca astrociti, cellule gliali di Bergmann e condrociti di cartilagine elastica in colorazioni immunoistochimiche. L′anticorpo reagisce con antigeni specifici delle cellule glia in sezioni di tessuto congelate o fissate in alcol.

The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Immunogeno

GFAP da midollo spinale porcino

GFAP from pig spinal cord

Applicazioni

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in

• immunohistochemistry
• immunoblotting
• immunolabelling,
• immunocytochemistry
• immunofluorescence

Azioni biochim/fisiol

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Note legali

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of Cytiva

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)