C3172
Creatininase from microorganisms
lyophilized powder, 100-300 units/mg protein
Sinonimo/i:
Creatinine Amidohydrolase
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About This Item
Prodotti consigliati
Forma fisica
lyophilized powder
Livello qualitativo
Attività specifica
100-300 units/mg protein
PM
~175 kDa
Composizione
Protein, 65-85%
Attività estranea
Creatinase and urease ≤1%
Hexokinase and ATPase ≤0.1%
Temperatura di conservazione
2-8°C
Applicazioni
Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine.
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Azioni biochim/fisiol
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Proprietà fisiche
Isoelectric point:4.7
Michaelis constants:3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)
Structure:6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors:Ag+, Hg++, N-bromosuccinimide, EDTA
Optimum pH:6.5 − 7.5
Optimum temp:70°C
pH Stability:pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability:Below 70°C (pH 7.5, 30 min)
Michaelis constants:3.2 x 10‾2M (Creatinine), 5.7 x 10‾2M (Creatine)
Structure:6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors:Ag+, Hg++, N-bromosuccinimide, EDTA
Optimum pH:6.5 − 7.5
Optimum temp:70°C
pH Stability:pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability:Below 70°C (pH 7.5, 30 min)
Definizione di unità
One unit will hydrolyze 1.0 μmole of creatinine to creatine per min at pH 8.0 and 25 °C
Stato fisico
Lyophilized powder containing sucrose and BSA as stabilizers
Risultati analitici
Protein determined by biuret.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificati d'analisi (COA)
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Journal of molecular biology, 337(2), 399-416 (2004-03-09)
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Acta biomaterialia, 1(2), 193-199 (2006-05-17)
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Protocolli
Assay Procedure for Creatininase
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