C3138

Cathepsin D from bovine spleen

lyophilized powder, ≥2.0 units/mg protein

• Sinonimo/i: CTSD
• Numero CAS: 9025-26-7
• Classificazione EC (Enzyme Commission): 3.4.23.5 (BRENDA, IUBMB)
• Numero MDL: MFCD00130749
• Codice UNSPSC: 12352204
• NACRES: NA.32

Proprietà

• Origine biologica: bovine spleen
• Saggio: 10—70% protein (biuret)
• Forma fisica: lyophilized powder
• Attività specifica: ≥2.0 units/mg protein
• PM: ~45 kDa
• Produttore/marchio commerciale: Sigma-Aldrich
• tecniche: activity assay: suitable
• Colore: dark brown
• Compatibilità: suitable for molecular biology
• N° accesso UniProt: P80209
• applicazioni: life science and biopharma
• Temperatura di conservazione: −20°C
• Informazioni sul gene: cow ... CTSD(282883)

Descrizione

Descrizione generale

Research area: Cell Signaling

Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.

Applicazioni

Cathepsin D from bovine spleen has been used:

• in in vitro dose-dependent fluorometric activity assays.
• in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
• for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
• in cathepsin D activity assay.

Azioni biochim/fisiol

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Definizione di unità

One unit will produce an increase in A₂₈₀ of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).

Stato fisico

Lyophilized powder containing citrate buffer salts

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable