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Key Documents

C0150

Sigma-Aldrich

Cathepsin B from human placenta

lyophilized powder, ≥5 units/mg protein

Sinonimo/i:

Cathepsin B1

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Forma fisica

lyophilized powder

Livello qualitativo

Attività specifica

≥5 units/mg protein

PM

24.5 kDa

Composizione

Protein, ~50% Lowry

Solubilità

H2O: soluble 1.0 mg/mL, clear

N° accesso UniProt

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CTSB(1508)

Descrizione generale

Cathepsin B is encoded by the gene CTSB and is located in the human chromosome at 8p23.1. Cathepsin B is a lysosomal proteolytic enzyme of cysteine protease family. It is present in all mammalian cells. It is essential for the intracellular protein turnover.

Applicazioni

Cathepsin B from human placenta has been used in the in vitro digestion of modified myelin oligodendrocyte glycoprotein (MOG). It has also been used in determining the effect of cysteine protease inhibitors.
Cathepsin B is a lysosomal cysteine proteinase which hydrolyzes proteins with a broad specificity for peptide bonds. Cathepsin B may be a useful tool in Alzheimer′s research, as it may have a role in the natural defense against the disease . Cathepsin B may be used to cleave procaspase 1 and procaspase 11, and to induce apoptosis in digitonin-permeabilized cells.

Azioni biochim/fisiol

Cathepsin B preferentially cleaves at the caboxyl side of Arg-Arg bonds in small molecule substrates. Cathepsin B has been shown to degrade β-amyloid precursor protein into harmless fragments . Cathepsin B inhibition has been shown to limit bone metastasis in breast cancer .
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Definizione di unità

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Stato fisico

Lyophilized powder containing phosphate buffer salts

Nota sulla preparazione

Dissolves in water at 1 mg/mL concentration, yieliding a clear solution.

Inibitore

N° Catalogo
Descrizione
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Substrato

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Descrizione
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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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European Journal of Cancer, 35(1), 138-144 (1999)
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Articoli

Enzymatic Assay of Cathepsin B

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