B9673

1-Bromo-3-chloropropane

for isolation of RNA

• Sinonimo/i: 1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide
• Formula condensata: Cl(CH₂)₃Br
• Numero CAS: 109-70-6
• Peso molecolare: 157.44
• Beilstein: 605278
• Numero CE: 203-697-1
• Numero MDL: MFCD00000998
• Codice UNSPSC: 41105515
• ID PubChem: 24892149
• NACRES: NA.52

Proprietà

• Grado: for molecular biology
• Saggio: ≥98.5% (GC)
• Forma fisica: liquid
• Indice di rifrazione: n20/D 1.486 (lit.)
• P. eboll.: 144-145 °C (lit.)
• Densità: 1.592 g/mL at 25 °C (lit.)
• Temperatura di conservazione: room temp
• Stringa SMILE: ClCCCBr
• InChI: 1S/C3H6BrCl/c4-2-1-3-5/h1-3H2; MFESCIUQSIBMSM-UHFFFAOYSA-N

Descrizione

Descrizione generale

BCP can be used in place of chloroform and is less toxic. The use of BCP in the single-step TRI reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA. 1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Applicazioni

1-Bromo-3-chloropropane has been used in molecular biology applications for the isolation of high quality RNA from:

• feline teeth
• mice brain and liver tissues
• human ectocervical epithelial cells
• placental samples
• human mesenchymal stromal cells (hMSCs)

Caratteristiche e vantaggi

• less toxic alternative to chloroform
• compatible with TRI reagent for isolation of RNA with decreased DNA contamination

Altre note

BCP can be used in place of chloroform, and is less toxic. The use of BCP in the single-step TRI Reagent method decreases the possibility of contaminating RNA with DNA. It does not adversely affect quality or quantity of the isolated RNA.

Informazioni sulla sicurezza

• Pittogrammi: GHS06,GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H302,H331,H335,H341,H412
• Consigli di prudenza: P202 - P261 - P273 - P301 + P312 - P304 + P340 + P311 - P308 + P313
• Classi di pericolo: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Muta. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Dispositivi di protezione individuale: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter