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C7559

Sigma-Aldrich

Cloroformio

≥99%, PCR Reagent, contains amylenes as stabilizer

Sinonimo/i:

Triclorometano, Tricloruro di metilidina

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About This Item

Formula empirica (notazione di Hill):
CHCl3
Numero CAS:
Peso molecolare:
119.38
Beilstein:
1731042
Numero CE:
Numero MDL:
Codice UNSPSC:
12191502
ID PubChem:

Grado

PCR Reagent

Livello qualitativo

Densità del vapore

4.1 (vs air)

Tensione di vapore

160 mmHg ( 20 °C)

Saggio

≥99%

Forma fisica

liquid

contiene

amylenes as stabilizer

Confezionamento

vial of 1.5 mL

tecniche

PCR: suitable

Colore

colorless

Indice di rifrazione

n20/D 1.445 (lit.)

P. eboll.

60.5-61.5 °C (lit.)

Punto di fusione

−63 °C (lit.)

Densità

1.492 g/mL at 25 °C (lit.)

Attività estranea

DNase, RNase, none detected

Stringa SMILE

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H
HEDRZPFGACZZDS-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.

Applicazioni

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification.

Pittogrammi

Skull and crossbonesHealth hazard

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Organi bersaglio

Central nervous system

Codice della classe di stoccaggio

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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