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A9169

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Anti Rabbit Antibody - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, Anti Rabbit Antibody Sigma

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About This Item

Numero MDL:
MFCD00162788
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Livello qualitativo

300

Coniugato

peroxidase conjugate

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

rabbit

tecniche

direct ELISA: 1:50,000 using using 5 μg/ml of rabbit IgG for coating and OPD substrate
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400
western blot (chemiluminescent): 1:80,000-1:160,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per well)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Categorie correlate

Descrizione generale

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge.
Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody binds all rabbit Igs.

Specificità

Specificity of the Peroxidase Conjugated Anti-Rabbit IgG antibodies is determined by immunoelectrophoresis (IEP) versus normal rabbit serum and rabbit IgG.

Immunogeno

purified rabbit IgG

Applicazioni

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used for immunoblot assays at 1:1,000. The antibody can also be used for ELISA (1:5,000) and IHC (1:400) applications.
Cell-associated protein samples from E. coli cultures were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Azioni biochim/fisiol

Immunoglobulins are glycoprotein antibodies that modulate several immune responses. Immunoglobulin G participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Nota sulla preparazione

Prepared by two-step glutaraldehyde method.

Stoccaggio e stabilità

For continuous use, store at 0-5EC. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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