12-348
Goat Anti-Rabbit IgG Antibody, HRP-conjugate
1 mg/mL, Upstate®
Sinonimo/i:
Anti-rabbit IgG
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Prodotti consigliati
Origine biologica
goat
Livello qualitativo
Coniugato
peroxidase conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Reattività contro le specie
rabbit
Produttore/marchio commerciale
Upstate®
Concentrazione
1 mg/mL
tecniche
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Specificità
Specific for rabbit IgG heavy and light chains.
Immunogeno
Highly purified whole rabbit IgG.
Applicazioni
Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA.
Qualità
Evaluated by western blot.
Western Blot Analysis: Suitable for western blotting (dot blot), ELISA and immunohistochemistry. A working dilution of 1:15,000 to 1:60,000 is suggested for ELISA. A working dilution of 1:2,000 to 1:6,000 is suggested for western blotting procedures. A working dilution of 1:500 to 1:3,000 is suggested for immunohistochemistry. Optimal antibody dilution for other applications should be determined by the researcher.
Western Blot Analysis: Suitable for western blotting (dot blot), ELISA and immunohistochemistry. A working dilution of 1:15,000 to 1:60,000 is suggested for ELISA. A working dilution of 1:2,000 to 1:6,000 is suggested for western blotting procedures. A working dilution of 1:500 to 1:3,000 is suggested for immunohistochemistry. Optimal antibody dilution for other applications should be determined by the researcher.
Stato fisico
Goat polyclonal conjugated with horseradish peroxidase IgG in buffer containing 50% storage buffer 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, 0.01% (w/v) gentamicin sulfate and 50% glycerol.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
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