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A8792

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

HRP Rabbit Anti-Human IgG (whole molecule)

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

rabbit

Coniugato

peroxidase conjugate

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

buffered aqueous solution

Reattività contro le specie

human

tecniche

direct ELISA: 1:40,000
dot blot: 1:80,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG.

Specificità

Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Immunogeno

purified human IgG

Applicazioni

Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
enzyme-linked immunosorbent assay (ELISA)
western blotting
microarray analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

Azioni biochim/fisiol

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Stato fisico

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Nota sulla preparazione

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Molecular Genetics of Immunoglobulin, 17 (1987)
Rajika Lasanthi Dewasurendra et al.
BMC infectious diseases, 17(1), 49-49 (2017-01-11)
Sri Lanka achieved the WHO certificate as a malaria free country in September 2016, thus monitoring of malaria transmission using sensitive and effective tools is an important need. Use of age-specific antibody prevalence as a serological tool to predict transmission
Identification and clinical significance of an elevated level of serum aminoacylase-1 autoantibody in patients with hepatitis B virus-related liver cirrhosis
He X, et al.
Molecular Medicine Reports, 14(5), 4255-4262 (2016)
P A Maple et al.
Journal of clinical pathology, 54(10), 812-815 (2001-09-29)
A time resolved fluorometric immunoassay (TRFIA) has been developed and compared with an in house enzyme linked immunosorbent assay (ELISA) and commercial ELISA (Bindazyme) for the detection of tetanus antitoxin in human sera. A panel of 132 sera submitted for
Mercy Guech-Ongey et al.
International journal of cancer, 130(8), 1908-1914 (2011-06-02)
The role of protective immunity to Plasmodium falciparum (Pf) malaria in Burkitt lymphoma (BL) is unknown. We investigated the association between BL and antibodies reactive to SE36 antigen, a recombinant protein based on P. falciparum serine repeat antigen 5 gene

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