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Key Documents

A8706

Sigma-Aldrich

Avidin from egg white

recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Sinonimo/i:

AVID_CHICK

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About This Item

Numero CAS:
1405-69-2
Numero CE:
215-783-6
Numero MDL:
MFCD00130572
Codice UNSPSC:
12352202
NACRES:
NA.56

Ricombinante

expressed in corn

Saggio

≥80% protein basis (biuret)

Forma fisica

powder

Attività specifica

≥12 units/mg protein (E1%/280)

PM

glycoprotein 66 kDa
subunit 16 kDa

tecniche

ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable

N° accesso UniProt

P02701

Temperatura di conservazione

2-8°C

Informazioni sul gene

chicken ... AVD(396260)

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Descrizione generale

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Applicazioni

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.

Definizione di unità

One unit will bind 1.0 μg of d-biotin.

Nota sulla preparazione

Affinity purified

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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A new construction of a test-system for free thyroid hormone 3,3',5-triiodothyronine determination (free T3) in human blood serum in ELISA and IRMA variants was described. For this purpose a low-molecular weight bifunctional conjugate containing T3 and vitamin H (biotin, Bt)
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