A7005

β-Amylase from sweet potato

Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

• Sinonimo/i: 1,4-α-D-Glucan maltohydrolase
• Numero CAS: 9000-91-3
• Classificazione EC (Enzyme Commission): 3.2.1.2 (BRENDA, IUBMB)
• Numero CE: 232-566-1
• Numero MDL: MFCD00081391
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Origine biologica: sweet potato
• Livello qualitativo: 200
• Tipo: Type I-B
• Forma fisica: ammonium sulfate suspension
• Attività specifica: ≥750 units/mg protein (E1%/280)
• Punteggio alternativa più verde: old score: 57; new score: 1; Find out more about DOZN™ Scoring
• Caratteristiche più verdi: Waste Prevention; Safer Solvents and Auxiliaries; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Concentrazione: ≥7.0 mg/mL
• Categoria alternativa più verde: , Re-engineered
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

β-Amylases belongs to the class of amylolytic enzymes.

We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Waste Prevention”, “Safer Solvents and Auxiliaries” and “Enhanced Energy Efficiency”. Click here to view its DOZN scorecard.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Applicazioni

β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms. The enzyme has also been used to prepare β-limit dextrin from waxy maize starch.

β-Amylase from sweet potato has been used:

• in the hydrolysis of starch and starch-pectin combinations
• for comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assay
• for selenium speciation in samples

Azioni biochim/fisiol

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.

β-Amylases plays a major role in examining enzymatic promiscuity among glycosidases.

Definizione di unità

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.

Stato fisico

Crystalline suspension in 2.3 M (NH₄)₂SO₄

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P342 + P311
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3