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A8781

Sigma-Aldrich

β-Amylase from sweet potato

greener alternative

Sinonimo/i:

1,4-α-D-Glucan maltohydrolase

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About This Item

Numero CAS:
9000-91-3
Classificazione EC (Enzyme Commission):
3.2.1.2 (BRENDA, IUBMB)
Numero CE:
232-566-1
Numero MDL:
MFCD00081391
Codice UNSPSC:
41106514
NACRES:
NA.56

Forma fisica

powder

Livello qualitativo

100

PM

~200,000

Confezionamento

vial of 15 mg

Punteggio alternativa più verde

old score: 57
new score: 1
Find out more about DOZN™ Scoring

Caratteristiche più verdi

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

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Categoria alternativa più verde

Re-engineered,

Temperatura di conservazione

−20°C

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Categorie correlate

Descrizione generale

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.
We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Waste Prevention”, “Safer Solvents and Auxiliaries” and “Design for Energy Efficiency”. Click here to view its DOZN scorecard.

Applicazioni

β-Amylase from sweet potato has been used:
  • as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)
  • as a standard for calibration curve preparation in size exclusion chromatography
  • as a model protein for small-angle X-ray analysis (SAXs)

Azioni biochim/fisiol

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

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beta-amylases are used in production of maltose syrup. It is shown that sweet potato beta-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band
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